Hotspot

rps15-ycf1

SSC · 119719-120114 · 396 bp

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Pi 0.376
MarkerSeek score 77.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
396
variable_sites
129
pi
0.376
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT GAACCAGTTCGCCTGTCTAG 45.0 55.0 58.1 58.0 1377 bp 0.5 59.5
rps15-ycf1_p2 TAATCGTTGCCGTTTTCCCA GAACCAGTTCGCCTGTCTAG 45.0 55.0 58.1 58.0 1119 bp 0.5 59.1
rps15-ycf1_p3 AACACCATGCATTGATGGGT TTATCCCCTAGGCGTCGTAG 45.0 55.0 58.1 58.1 1152 bp 0.5 58.7
rps15-ycf1_p4 TCAACCGATCCTGGGTTTTC GAACCAGTTCGCCTGTCTAG 50.0 55.0 57.8 58.0 998 bp 0.5 58.4
rps15-ycf1_p5 CAACCGATCCTGGGTTTTCT GAACCAGTTCGCCTGTCTAG 50.0 55.0 57.8 58.0 997 bp 0.5 58.4