Hotspot

ndhG-ndhI

SSC · 114812-115272 · 461 bp

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Pi 0.449
MarkerSeek score 77.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
461
variable_sites
203
pi
0.449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AACATTAATAGCGCCCACGT AATCAAATTGCTTTGGGCCG 45.0 45.0 58.0 57.9 752 bp 0.5 59.0
ndhG-ndhI_p2 AATCATGTAGTGGTTCGGGC AATCAAATTGCTTTGGGCCG 50.0 45.0 58.0 57.9 564 bp 0.5 58.9
ndhG-ndhI_p3 AACATTAATAGCGCCCACGT CGACTTATGATCGCCACGAA 45.0 50.0 58.0 58.2 781 bp 0.5 58.4
ndhG-ndhI_p4 AACATTAATAGCGCCCACGT TTCGACTTATGATCGCCACG 45.0 50.0 58.0 58.2 783 bp 0.5 58.4
ndhG-ndhI_p5 AATCATGTAGTGGTTCGGGC CGACTTATGATCGCCACGAA 50.0 50.0 58.0 58.2 593 bp 0.5 58.4