Hotspot

rpl32-trnL-UAG

SSC · 109692-110147 · 456 bp

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Pi 0.408
MarkerSeek score 79.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
456
variable_sites
171
pi
0.408
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGTTCCCAAAAAGCGTAC GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.0 58.1 1135 bp 0.5 58.9
rpl32-trnL-UAG_p2 GCAGTTCCCAAAAAGCGTAC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 654 bp 0.5 58.6
rpl32-trnL-UAG_p3 GCAGTTCCCAAAAAGCGTAC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 647 bp 0.5 58.1
rpl32-trnL-UAG_p4 GCAGTTCCCAAAAAGCGTAC AAGACCCGAAGTAGCAAAGC 50.0 50.0 58.0 58.2 1142 bp 0.5 58.1
rpl32-trnL-UAG_p5 GCAGTTCCCAAAAAGCGTAC CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 652 bp 0.5 58.1