Hotspot

ndhF-rpl32

SSC · 109014-109529 · 516 bp

Back to Apios

Pi 0.421
MarkerSeek score 79.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
516
variable_sites
185
pi
0.421
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GATTTAGCGACTGAGCCACA GTACGCTTTTTGGGAACTGC 50.0 50.0 58.0 58.0 1261 bp 0.5 59.5
ndhF-rpl32_p2 GATTTAGCGACTGAGCCACA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1895 bp 0.5 58.9
ndhF-rpl32_p3 GATTTAGCGACTGAGCCACA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1888 bp 0.5 58.4
ndhF-rpl32_p4 ACATGAAGAGGGAATTGGGC GTACGCTTTTTGGGAACTGC 50.0 50.0 57.9 58.0 1282 bp 0.5 58.4
ndhF-rpl32_p5 GATTTAGCGACTGAGCCACA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1893 bp 0.5 58.3