Hotspot

ycf4

LSC · 42345-42902 · 558 bp

Back to Aphyllon

Pi 0.125

MarkerSeek score 82.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 97
pi: 0.125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GGATCGCCGGATGCATAAAA	CCTGCTGAGAAAACCTTCGT	50.0	50.0	58.8	58.1	846 bp	0.333333	46.3
ycf4_p2	GGATCGCCGGATGCATAAAA	CCGCATCGCACCTAAAGTAT	50.0	50.0	58.8	58.1	1143 bp	0.333333	46.1
ycf4_p3	GGATCGCCGGATGCATAAAA	TATCCGCATCGCACCTAAAG	50.0	50.0	58.8	57.9	1146 bp	0.333333	46.0
ycf4_p4	AAAGTAGATCAGGATCGCCG	CCTGCTGAGAAAACCTTCGT	50.0	50.0	57.2	58.1	857 bp	0.333333	46.0
ycf4_p5	GGATCGCCGGATGCATAAAA	TCCTGCTGAGAAAACCTTCG	50.0	50.0	58.8	57.8	847 bp	0.333333	45.9