Hotspot

rps16

LSC · 5372-6465 · 1094 bp

Back to Aphyllon

Pi 0.088

MarkerSeek score 83.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1094
variable_sites: 137
pi: 0.088
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TACAAGTTGCTACCCCCTTG	TCTGCGATAGACCCTAACGT	50.0	50.0	57.1	58.0	1719 bp	0.333333	46.0
rps16_p2	TACAAGTTGCTACCCCCTTG	TATGGACAGCTGAACCAACG	50.0	50.0	57.1	57.9	1201 bp	0.333333	45.6
rps16_p3	TACAAGTTGCTACCCCCTTG	CCCCATACCGGTTCCAAATT	50.0	50.0	57.1	57.8	1153 bp	0.333333	45.1
rps16_p4	CAAGTTGCTACCCCCTTGG	TCTGCGATAGACCCTAACGT	57.9	50.0	58.0	58.0	1717 bp	0.333333	44.6
rps16_p5	CAAGTTGCTACCCCCTTGG	TATGGACAGCTGAACCAACG	57.9	50.0	58.0	57.9	1199 bp	0.333333	44.3