Hotspot

ndhJ

LSC · 32755-33234 · 480 bp

Back to Aphyllon

Pi 0.156

MarkerSeek score 81.50

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 480
variable_sites: 103
pi: 0.156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	CGTGGTACAAGGTTCGTGAT	AAATCATGATGAGTCCGCCC	50.0	50.0	57.9	58.0	1174 bp	0.333333	52.5
ndhJ_p2	CGTGGTACAAGGTTCGTGAT	GGCCATTAATCTTCGGAGGG	50.0	55.0	57.9	58.1	1285 bp	0.333333	51.9
ndhJ_p3	CGTGGTACAAGGTTCGTGAT	GTCCGCCCCAAAGAGTTATT	50.0	50.0	57.9	57.9	1162 bp	0.333333	51.7
ndhJ_p4	CGTGGTACAAGGTTCGTGAT	ACCGCCATCTACTTCAGAGA	50.0	50.0	57.9	57.9	911 bp	0.333333	51.6
ndhJ_p5	TGGTACAAGGTTCGTGATGG	AAATCATGATGAGTCCGCCC	50.0	50.0	57.5	58.0	1172 bp	0.333333	49.4