Hotspot

atpI

LSC · 14227-14970 · 744 bp

Back to Aphyllon

Pi 0.0983

MarkerSeek score 81.30

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 95
pi: 0.0983
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TTCCATTGCTTGGTTCCGAA	AAGACTCAACCTCCTTCCGA	45.0	50.0	58.0	58.0	1674 bp	0.333333	53.2
atpI_p2	TTCCATTGCTTGGTTCCGAA	AGACTCAACCTCCTTCCGAA	45.0	50.0	58.0	58.0	1673 bp	0.333333	53.2
atpI_p3	TTCCATTGCTTGGTTCCGAA	TTGGTTTTTGGTGCAGCAAG	45.0	45.0	58.0	58.0	1880 bp	0.333333	53.1
atpI_p4	TTCCATTGCTTGGTTCCGAA	ATATATGGCCCTGCGAGAGT	45.0	50.0	58.0	58.1	1539 bp	0.333333	52.8
atpI_p5	TTCCATTGCTTGGTTCCGAA	TGGAACAAAAAGCGGGAAGA	45.0	45.0	58.0	57.9	1690 bp	0.333333	52.8