Hotspot

atpB

LSC · 35503-37002 · 1500 bp

Back to Aphyllon

Pi 0.0832

MarkerSeek score 81.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 150
pi: 0.0832
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CCCTTCGGTTTTCTTTGGGA	AACTGATCAACGTACCGTCG	50.0	50.0	58.0	58.0	2282 bp	0.333333	53.0
atpB_p2	TCCCTTCGGTTTTCTTTGGG	AACTGATCAACGTACCGTCG	50.0	50.0	58.0	58.0	2283 bp	0.333333	53.0
atpB_p3	CATACGGTGGGAGTCATTGG	AACTGATCAACGTACCGTCG	55.0	50.0	58.0	58.0	2220 bp	0.333333	52.7
atpB_p4	CCCTTCGGTTTTCTTTGGGA	TGATTGAATGGCACCAACGA	50.0	45.0	58.0	58.1	2335 bp	0.333333	52.5
atpB_p5	TCCCTTCGGTTTTCTTTGGG	TGATTGAATGGCACCAACGA	50.0	45.0	58.0	58.1	2336 bp	0.333333	52.5