Hotspot

PS16_p038

LSC · 1-51689 · 51689 bp

Back to Aphyllon

Pi 0.109
MarkerSeek score 69.10
Primer available yes
Label PS16_p038

Feature Metadata

label_name
PS16_p038
feature_type
gene
region
LSC
length_bp
51689
variable_sites
5393
pi
0.109
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.73
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
PS16_p038_p1 TGGGTTAGGGAGATGCAGAA TTGCGGGTTAGTTATTCGGG 50.0 50.0 58.0 58.0 1742 bp 0.333333 52.8
PS16_p038_p2 TTGGGTTAGGGAGATGCAGA TTGCGGGTTAGTTATTCGGG 50.0 50.0 58.0 58.0 1743 bp 0.333333 52.8
PS16_p038_p3 TGGGTTAGGGAGATGCAGAA GCGGGTTAGTTATTCGGGTT 50.0 50.0 58.0 58.0 1740 bp 0.333333 52.8
PS16_p038_p4 TTGGGTTAGGGAGATGCAGA GCGGGTTAGTTATTCGGGTT 50.0 50.0 58.0 58.0 1741 bp 0.333333 52.8
PS16_p038_p5 TTTCCCCATTCCCCACTACT TTGCGGGTTAGTTATTCGGG 50.0 50.0 57.9 58.0 2051 bp 0.333333 52.6