Hotspot

PS16_r008-trnA-UGC

SSC · 79370-79915 · 546 bp

Back to Aphyllon

Pi 0.0565

MarkerSeek score 81.60

Primer available yes

Label PS16_r008-trnA-UGC

Feature Metadata

label_name: PS16_r008-trnA-UGC
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 44
pi: 0.0565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PS16_r008-trnA-UGC_p1	CAAGGTAGCCGTACTGGAAG	CTCGAACCGCTGACATCC	55.0	61.1	57.7	57.9	608-2306 bp	1.0	64.3
PS16_r008-trnA-UGC_p2	AGTGACTGGAGTGAAGTCGT	CTCGAACCGCTGACATCC	50.0	61.1	58.3	57.9	630-2328 bp	1.0	64.2
PS16_r008-trnA-UGC_p3	AAGTCGTAACAAGGTAGCCG	CTCGAACCGCTGACATCC	50.0	61.1	57.6	57.9	617-2315 bp	1.0	63.8
PS16_r008-trnA-UGC_p4	AACAAGGTAGCCGTACTGGA	CTCGAACCGCTGACATCC	50.0	61.1	58.4	57.9	610-2308 bp	1.0	63.8
PS16_r008-trnA-UGC_p5	ACAAGGTAGCCGTACTGGAA	CTCGAACCGCTGACATCC	50.0	61.1	58.4	57.9	609-2307 bp	1.0	63.8