Hotspot

psaB-trnS-GGA

LSC · 28274-28923 · 650 bp

Back to Aphyllon

Pi 0.295

MarkerSeek score 84.40

Primer available yes

Label psaB-trnS-GGA

Feature Metadata

label_name: psaB-trnS-GGA
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 273
pi: 0.295
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-trnS-GGA_p1	GTGCACTACTCCGTGGTAAG	TGAAAGGCCTAACTGAGGGA	55.0	50.0	58.0	58.0	1189 bp	0.333333	53.0
psaB-trnS-GGA_p2	GCTACCAAACTGCTCTACCC	TGAAAGGCCTAACTGAGGGA	55.0	50.0	58.0	58.0	1504 bp	0.333333	52.8
psaB-trnS-GGA_p3	AAGGTTATGAGCCTTGCGAG	TGAAAGGCCTAACTGAGGGA	50.0	50.0	58.0	58.0	1523 bp	0.333333	52.8
psaB-trnS-GGA_p4	GATGTTGCGGAGACAGGATT	TGAAAGGCCTAACTGAGGGA	50.0	50.0	58.0	58.0	1558 bp	0.333333	52.8
psaB-trnS-GGA_p5	GTGCACTACTCCGTGGTAAG	GGGGTCAACAGGTCAAGTTT	55.0	50.0	58.0	57.9	1768 bp	0.333333	52.7