primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
PS16_p038_p1	PS16_p038	1	TGGGTTAGGGAGATGCAGAA	TTGCGGGTTAGTTATTCGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061589	45821	46820	1742-1742	0.333333	52.8
PS16_p038_p2	PS16_p038	2	TTGGGTTAGGGAGATGCAGA	TTGCGGGTTAGTTATTCGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061589	45821	46820	1743-1743	0.333333	52.8
PS16_p038_p3	PS16_p038	3	TGGGTTAGGGAGATGCAGAA	GCGGGTTAGTTATTCGGGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063006	45821	46820	1740-1740	0.333333	52.8
PS16_p038_p4	PS16_p038	4	TTGGGTTAGGGAGATGCAGA	GCGGGTTAGTTATTCGGGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063006	45821	46820	1741-1741	0.333333	52.8
PS16_p038_p5	PS16_p038	5	TTTCCCCATTCCCCACTACT	TTGCGGGTTAGTTATTCGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.095835	45821	46820	2051-2051	0.333333	52.6
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCACGATCATGAGCAAATGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.9	58.2	12.1	17.1	0.283768	3696	4397	832-833	0.666667	73.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCACGATCATGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.9	58.2	12.1	17.1	0.283768	3696	4397	831-832	0.666667	73.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	GATTTCCGCATGGACAGAGT	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.198258	3696	4397	1212-1212	0.333333	51.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	GATTTCCGCATGGACAGAGT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.0	58.2	0.0	17.1	0.198258	3696	4397	1211-1211	0.333333	51.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	GATTTCCGCATGGACAGAGT	TCGCGGTTTTATTAGCTCGT	20	20	50.0	45.0	58.0	57.7	0.0	6.1	0.312539	3696	4397	1364-1364	0.333333	50.8
rps16_p1	rps16	1	TACAAGTTGCTACCCCCTTG	TCTGCGATAGACCCTAACGT	20	20	50.0	50.0	57.1	58.0	1.8	0.0	0.919541	5372	6465	1719-1719	0.333333	46.0
rps16_p2	rps16	2	TACAAGTTGCTACCCCCTTG	TATGGACAGCTGAACCAACG	20	20	50.0	50.0	57.1	57.9	1.8	29.3	0.964403	5372	6465	1201-1201	0.333333	45.6
rps16_p3	rps16	3	TACAAGTTGCTACCCCCTTG	CCCCATACCGGTTCCAAATT	20	20	50.0	50.0	57.1	57.8	1.8	15.0	1.024130	5372	6465	1153-1153	0.333333	45.1
rps16_p4	rps16	4	CAAGTTGCTACCCCCTTGG	TCTGCGATAGACCCTAACGT	19	20	57.9	50.0	58.0	58.0	0.0	0.0	1.087020	5372	6465	1717-1717	0.333333	44.6
rps16_p5	rps16	5	CAAGTTGCTACCCCCTTGG	TATGGACAGCTGAACCAACG	19	20	57.9	50.0	58.0	57.9	0.0	29.3	1.131882	5372	6465	1199-1199	0.333333	44.3
trnG-UCC_p1	trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TTCAGGAACAAATGGACCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.100296	9253	9948	1538-1848	0.666667	77.9
trnG-UCC_p2	trnG-UCC	2	CAATCCGACGCTTTAGTCCA	ATTGGAACTGGCGCAATTTG	20	20	50.0	45.0	58.0	57.9	0.0	10.8	0.087749	9253	9948	1682-2177	0.666667	77.6
trnG-UCC_p3	trnG-UCC	3	AATCCGACGCTTTAGTCCAC	ATTGGAACTGGCGCAATTTG	20	20	50.0	45.0	58.0	57.9	0.0	10.8	0.089137	9253	9948	1681-2176	0.666667	77.6
trnG-UCC_p4	trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088077	9253	9948	1879-1879	0.333333	52.6
trnG-UCC_p5	trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089465	9253	9948	1878-1878	0.333333	52.6
atpI_p1	atpI	1	TTCCATTGCTTGGTTCCGAA	AAGACTCAACCTCCTTCCGA	20	20	45.0	50.0	58.0	58.0	6.2	0.0	0.022829	14227	14970	1674-1674	0.333333	53.2
atpI_p2	atpI	2	TTCCATTGCTTGGTTCCGAA	AGACTCAACCTCCTTCCGAA	20	20	45.0	50.0	58.0	58.0	6.2	0.0	0.022829	14227	14970	1673-1673	0.333333	53.2
atpI_p3	atpI	3	TTCCATTGCTTGGTTCCGAA	TTGGTTTTTGGTGCAGCAAG	20	20	45.0	45.0	58.0	58.0	6.2	28.2	0.033062	14227	14970	1880-1880	0.333333	53.1
atpI_p4	atpI	4	TTCCATTGCTTGGTTCCGAA	ATATATGGCCCTGCGAGAGT	20	20	45.0	50.0	58.0	58.1	6.2	0.0	0.067529	14227	14970	1539-1539	0.333333	52.8
atpI_p5	atpI	5	TTCCATTGCTTGGTTCCGAA	TGGAACAAAAAGCGGGAAGA	20	20	45.0	45.0	58.0	57.9	6.2	0.0	0.071757	14227	14970	1690-1690	0.333333	52.8
psaB-trnS-GGA_p1	psaB-trnS-GGA	1	GTGCACTACTCCGTGGTAAG	TGAAAGGCCTAACTGAGGGA	20	20	55.0	50.0	58.0	58.0	10.3	5.2	0.046693	28274	28923	1189-1189	0.333333	53.0
psaB-trnS-GGA_p2	psaB-trnS-GGA	2	GCTACCAAACTGCTCTACCC	TGAAAGGCCTAACTGAGGGA	20	20	55.0	50.0	58.0	58.0	0.0	5.2	0.064451	28274	28923	1504-1504	0.333333	52.8
psaB-trnS-GGA_p3	psaB-trnS-GGA	3	AAGGTTATGAGCCTTGCGAG	TGAAAGGCCTAACTGAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	5.2	0.068701	28274	28923	1523-1523	0.333333	52.8
psaB-trnS-GGA_p4	psaB-trnS-GGA	4	GATGTTGCGGAGACAGGATT	TGAAAGGCCTAACTGAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	5.2	0.068701	28274	28923	1558-1558	0.333333	52.8
psaB-trnS-GGA_p5	psaB-trnS-GGA	5	GTGCACTACTCCGTGGTAAG	GGGGTCAACAGGTCAAGTTT	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.075196	28274	28923	1768-1768	0.333333	52.7
ndhJ_p1	ndhJ	1	CGTGGTACAAGGTTCGTGAT	AAATCATGATGAGTCCGCCC	20	20	50.0	50.0	57.9	58.0	0.0	9.5	0.101675	32755	33234	1174-1174	0.333333	52.5
ndhJ_p2	ndhJ	2	CGTGGTACAAGGTTCGTGAT	GGCCATTAATCTTCGGAGGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.176987	32755	33234	1285-1285	0.333333	51.9
ndhJ_p3	ndhJ	3	CGTGGTACAAGGTTCGTGAT	GTCCGCCCCAAAGAGTTATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.198824	32755	33234	1162-1162	0.333333	51.7
ndhJ_p4	ndhJ	4	CGTGGTACAAGGTTCGTGAT	ACCGCCATCTACTTCAGAGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.219944	32755	33234	911-911	0.333333	51.6
ndhJ_p5	ndhJ	5	TGGTACAAGGTTCGTGATGG	AAATCATGATGAGTCCGCCC	20	20	50.0	50.0	57.5	58.0	0.0	9.5	0.492988	32755	33234	1172-1172	0.333333	49.4
atpB_p1	atpB	1	CCCTTCGGTTTTCTTTGGGA	AACTGATCAACGTACCGTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037155	35503	37002	2282-2282	0.333333	53.0
atpB_p2	atpB	2	TCCCTTCGGTTTTCTTTGGG	AACTGATCAACGTACCGTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037155	35503	37002	2283-2283	0.333333	53.0
atpB_p3	atpB	3	CATACGGTGGGAGTCATTGG	AACTGATCAACGTACCGTCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.073303	35503	37002	2220-2220	0.333333	52.7
atpB_p4	atpB	4	CCCTTCGGTTTTCTTTGGGA	TGATTGAATGGCACCAACGA	20	20	50.0	45.0	58.0	58.1	0.0	6.3	0.107356	35503	37002	2335-2335	0.333333	52.5
atpB_p5	atpB	5	TCCCTTCGGTTTTCTTTGGG	TGATTGAATGGCACCAACGA	20	20	50.0	45.0	58.0	58.1	0.0	6.3	0.107356	35503	37002	2336-2336	0.333333	52.5
ycf4_p1	ycf4	1	GGATCGCCGGATGCATAAAA	CCTGCTGAGAAAACCTTCGT	20	20	50.0	50.0	58.8	58.1	5.0	0.0	0.880944	42345	42902	846-846	0.333333	46.3
ycf4_p2	ycf4	2	GGATCGCCGGATGCATAAAA	CCGCATCGCACCTAAAGTAT	20	20	50.0	50.0	58.8	58.1	5.0	0.0	0.899314	42345	42902	1143-1143	0.333333	46.1
ycf4_p3	ycf4	3	GGATCGCCGGATGCATAAAA	TATCCGCATCGCACCTAAAG	20	20	50.0	50.0	58.8	57.9	5.0	0.0	0.914095	42345	42902	1146-1146	0.333333	46.0
ycf4_p4	ycf4	4	AAAGTAGATCAGGATCGCCG	CCTGCTGAGAAAACCTTCGT	20	20	50.0	50.0	57.2	58.1	3.8	0.0	0.918552	42345	42902	857-857	0.333333	46.0
ycf4_p5	ycf4	5	GGATCGCCGGATGCATAAAA	TCCTGCTGAGAAAACCTTCG	20	20	50.0	50.0	58.8	57.8	5.0	0.0	0.935011	42345	42902	847-847	0.333333	45.9
PS16_p039_p1	PS16_p039	1	GCTTGGCGATTCTAGAACCA	TTGTACGGGATGTAACCCCT	20	20	50.0	50.0	58.0	58.0	7.5	0.0	0.068725	56251	57050	641-1640	0.666667	75.8
PS16_p039_p2	PS16_p039	2	GCTTGGCGATTCTAGAACCA	AGAGACGCAGCATTAAGAGC	20	20	50.0	50.0	58.0	58.1	7.5	0.0	0.090929	56251	57050	690-1689	0.666667	75.4
PS16_p039_p3	PS16_p039	3	GCTTGGCGATTCTAGAACCA	GAGACGCAGCATTAAGAGCT	20	20	50.0	50.0	58.0	58.1	7.5	0.0	0.090929	56251	57050	689-1688	0.666667	75.4
PS16_p039_p4	PS16_p039	4	GCTTGGCGATTCTAGAACCA	AAAAGGTCCCGGTCTAGGAA	20	20	50.0	50.0	58.0	58.0	7.5	0.0	0.058612	56251	57050	1708-1708	0.333333	52.9
PS16_p039_p5	PS16_p039	5	GGTAAACCAGCGGATCCATT	AAAAGGTCCCGGTCTAGGAA	20	20	50.0	50.0	57.9	58.0	13.4	0.0	0.087219	56251	57050	1679-1679	0.333333	52.6
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021142	53775	54251	1052-1052	0.333333	53.2
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	CCCGCCTGTAATACTCCAAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.051486	53775	54251	796-796	0.333333	52.9
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CCGCCTGTAATACTCCAACC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.051486	53775	54251	795-795	0.333333	52.9
clpP-psbB_p4	clpP-psbB	4	TTTATCTTTCGGGGGCGAAG	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052871	53775	54251	1137-1137	0.333333	52.9
clpP-psbB_p5	clpP-psbB	5	CAAATCGCGGTGGTCTTTTC	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	6.1	0.0	0.056444	53775	54251	1499-1499	0.333333	52.9
PS16_r008-trnA-UGC_p1	PS16_r008-trnA-UGC	1	CAAGGTAGCCGTACTGGAAG	CTCGAACCGCTGACATCC	20	18	55.0	61.1	57.7	57.9	7.8	0.0	2.389936	79370	79915	608-2306	1.000000	64.3
PS16_r008-trnA-UGC_p2	PS16_r008-trnA-UGC	2	AGTGACTGGAGTGAAGTCGT	CTCGAACCGCTGACATCC	20	18	50.0	61.1	58.3	57.9	0.0	0.0	2.401057	79370	79915	630-2328	1.000000	64.2
PS16_r008-trnA-UGC_p3	PS16_r008-trnA-UGC	3	AAGTCGTAACAAGGTAGCCG	CTCGAACCGCTGACATCC	20	18	50.0	61.1	57.6	57.9	0.0	0.0	2.453623	79370	79915	617-2315	1.000000	63.8
PS16_r008-trnA-UGC_p4	PS16_r008-trnA-UGC	4	AACAAGGTAGCCGTACTGGA	CTCGAACCGCTGACATCC	20	18	50.0	61.1	58.4	57.9	7.8	0.0	2.458535	79370	79915	610-2308	1.000000	63.8
PS16_r008-trnA-UGC_p5	PS16_r008-trnA-UGC	5	ACAAGGTAGCCGTACTGGAA	CTCGAACCGCTGACATCC	20	18	50.0	61.1	58.4	57.9	7.8	0.0	2.458535	79370	79915	609-2307	1.000000	63.8