Hotspot

ndhF

SSC · 108810-111041 · 2232 bp

Back to Aphragmus

Pi 0.0527

MarkerSeek score 58.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 37
pi: 0.0527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.31
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGCTGAATTCCACGTTCGA	AGGTTTGAAATCACGTGCTT	45.0	40.0	57.9	56.2	2427 bp	0.5	44.6
ndhF_p2	CCACCGAAGAAGCTCCTTTT	AGGTTTGAAATCACGTGCTT	50.0	40.0	58.1	56.2	2538 bp	0.5	44.5
ndhF_p3	TGCTGAATTCCACGTTCGAA	AGGTTTGAAATCACGTGCTT	45.0	40.0	58.1	56.2	2426 bp	0.5	44.3
ndhF_p4	TATCCACCGAAGAAGCTCCT	AGGTTTGAAATCACGTGCTT	50.0	40.0	57.8	56.2	2541 bp	0.5	44.0
ndhF_p5	ACCGAAGAAGCTCCTTTTCC	AGGTTTGAAATCACGTGCTT	50.0	40.0	57.8	56.2	2536 bp	0.5	43.8