Hotspot

accD

LSC · 55572-57026 · 1455 bp

Back to Aphragmus

Pi 0.0364

MarkerSeek score 65.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 53
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGAACCAAGTATCCCCACCT	CCGGAAAGACTAGGCCTACT	50.0	55.0	57.9	58.2	2620 bp	0.5	57.8
accD_p2	TGCTCGGCTCAATCCTTTTT	CCGGAAAGACTAGGCCTACT	45.0	55.0	58.1	58.2	2849 bp	0.5	57.5
accD_p3	TTGCTCGGCTCAATCCTTTT	CCGGAAAGACTAGGCCTACT	45.0	55.0	58.1	58.2	2850 bp	0.5	57.5
accD_p4	TGAACCAAGTATCCCCACCT	CAAGTGCCGGAAAGACTAGG	50.0	55.0	57.9	58.3	2626 bp	0.5	57.4
accD_p5	ATTGCTCGGCTCAATCCTTT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.9	58.2	2851 bp	0.5	57.1