Hotspot

ccsA-ndhD

SSC · 113937-114447 · 511 bp

Back to Aphragmus

Pi 0.28

MarkerSeek score 79.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 511
variable_sites: 123
pi: 0.28
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCGGACCTGCTTTTTGGAAA	CCTTGGTTGATGGTAGTCGG	45.0	55.0	57.9	58.0	1365 bp	0.5	59.3
ccsA-ndhD_p2	TCGGACCTGCTTTTTGGAAA	AATTATTACCGCACGCCCAT	45.0	45.0	57.9	58.0	1397 bp	0.5	59.3
ccsA-ndhD_p3	TCGGACCTGCTTTTTGGAAA	ATTATTACCGCACGCCCATT	45.0	45.0	57.9	58.0	1396 bp	0.5	59.3
ccsA-ndhD_p4	CTCTCCAGTCCCAGTGGTTA	CCTTGGTTGATGGTAGTCGG	55.0	55.0	58.1	58.0	1479 bp	0.5	59.3
ccsA-ndhD_p5	CTCTCCAGTCCCAGTGGTTA	ATTATTACCGCACGCCCATT	55.0	45.0	58.1	58.0	1510 bp	0.5	59.3