Hotspot

psaJ-rpl33

LSC · 65170-65598 · 429 bp

Back to Aphragmus

Pi 0.0847

MarkerSeek score 75.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 36
pi: 0.0847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.0	57.9	1276-1307 bp	1.0	86.0
psaJ-rpl33_p2	CGAACTGAACTAAGAGCGCT	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.0	58.1	1288-1319 bp	1.0	86.0
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	TTCCAATCGACTAGGGGTGT	50.0	50.0	58.0	58.1	1289-1320 bp	1.0	85.9
psaJ-rpl33_p4	CGAACTGAACTAAGAGCGCT	TTTCTTGATTTCCCCGTGCA	50.0	45.0	58.0	58.0	1377 bp	0.5	59.8
psaJ-rpl33_p5	CGAACTGAACTAAGAGCGCT	CCCCGTGCATTGTATGGTTA	50.0	50.0	58.0	58.0	1366 bp	0.5	59.5