Hotspot

psbF-psbE

LSC · 62632-62640 · 9 bp

Back to Aphragmus

Pi 0.222

MarkerSeek score 77.50

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 2
pi: 0.222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	CGGTAGGTACAGCTAGTCCA	ACAGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	136 bp	1.0	80.8
psbF-psbE_p2	CGGTAGGTACAGCTAGTCCA	AACAGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	137 bp	1.0	80.7
psbF-psbE_p3	ACGGTAGGTACAGCTAGTCC	AACAGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	138 bp	1.0	80.7
psbF-psbE_p4	ACGGTAGGTACAGCTAGTCC	ACAGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	137 bp	1.0	80.7
psbF-psbE_p5	GCTAGTCCATGAACAGCCAA	ACAGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	125 bp	1.0	80.3