Hotspot

trnfM-AUG-rps14

LSC · 35929-36090 · 162 bp

Back to Aphragmus

Pi 0.0688

MarkerSeek score 76.40

Primer available yes

Label trnfM-AUG-rps14

Feature Metadata

label_name: trnfM-AUG-rps14
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 11
pi: 0.0688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-AUG-rps14_p1	GCTACCAAACTGCTCTACCC	TTCGGGAAATGGTTCATGCA	55.0	45.0	58.0	58.1	240-242 bp	1.0	83.4
trnfM-AUG-rps14_p2	AAGGTTATGAGCCTTGCGAG	TTCGGGAAATGGTTCATGCA	50.0	45.0	58.0	58.1	259-261 bp	1.0	83.1
trnfM-AUG-rps14_p3	GCTACCAAACTGCTCTACCC	TCGGGAAATGGTTCATGCAT	55.0	45.0	58.0	57.9	239-241 bp	1.0	83.0
trnfM-AUG-rps14_p4	TTTGAACCCGTGACCTCAAG	TTCGGGAAATGGTTCATGCA	50.0	45.0	58.0	58.1	276-278 bp	1.0	82.8
trnfM-AUG-rps14_p5	AAGGTTATGAGCCTTGCGAG	TCGGGAAATGGTTCATGCAT	50.0	45.0	58.0	57.9	258-260 bp	1.0	82.6