Hotspot

psbC-trnS-UCA

LSC · 34329-34507 · 179 bp

Back to Aphragmus

Pi 0.101

MarkerSeek score 78.10

Primer available yes

Label psbC-trnS-UCA

Feature Metadata

label_name: psbC-trnS-UCA
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 18
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UCA_p1	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	312 bp	1.0	81.1
psbC-trnS-UCA_p2	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	273 bp	1.0	80.3
psbC-trnS-UCA_p3	TTCGTGGGTCATTTATGGCA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.5	58.2	313 bp	1.0	79.1
psbC-trnS-UCA_p4	ATTCGTGGGTCATTTATGGCA	GGGTTCGAATCCCTCTCTCT	42.9	55.0	58.0	58.2	314 bp	1.0	75.0
psbC-trnS-UCA_p5	TCGTGGGTCATTTATGGCAC	TTCGAATCCCTCTCTCTCCT	50.0	50.0	58.3	56.9	309 bp	1.0	73.8