Hotspot

trnR-AGA-atpA

LSC · 9400-9693 · 294 bp

Back to Aphragmus

Pi 0.0692
MarkerSeek score 73.00
Primer available yes
Label trnR-AGA-atpA

Feature Metadata

label_name
trnR-AGA-atpA
feature_type
igs
region
LSC
length_bp
294
variable_sites
18
pi
0.0692
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-AGA-atpA_p1 GCGAAAAGCGTCCATTGTC GGAACGTTTTCTACTTCAGGAGA 52.6 43.5 58.0 58.1 378-404 bp 1.0 54.7
trnR-AGA-atpA_p2 GCGAAAAGCGTCCATTGTC GGAACGTTTTCTACTTCAGGAG 52.6 45.5 58.0 56.7 378-404 bp 1.0 53.6
trnR-AGA-atpA_p3 GCGAAAAGCGTCCATTGTC GGAACGTTTTCTACTTCAGGA 52.6 42.9 58.0 55.6 378-404 bp 1.0 52.2
trnR-AGA-atpA_p4 GCGAAAAGCGTCCATTGTCT GGAACGTTTTCTACTTCAGGAGA 50.0 43.5 59.5 58.1 378-404 bp 1.0 50.9
trnR-AGA-atpA_p5 GCGAAAAGCGTCCATTGTCT GGAACGTTTTCTACTTCAGGAG 50.0 45.5 59.5 56.7 378-404 bp 1.0 49.8