Hotspot

trnS-AGC-trnG-GGA

LSC · 7726-8386 · 661 bp

Back to Aphragmus

Pi 0.0583

MarkerSeek score 80.10

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 37
pi: 0.0583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	50.0	40.9	57.8	56.9	729-734 bp	1.0	60.9
trnS-AGC-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	50.0	40.9	58.2	56.9	727-732 bp	1.0	60.6
trnS-AGC-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCAC	50.0	43.5	57.8	58.2	729-734 bp	1.0	59.8
trnS-AGC-trnG-GGA_p4	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCAC	50.0	43.5	58.2	58.2	727-732 bp	1.0	59.5
trnS-AGC-trnG-GGA_p5	CCAACGCTTTAGTCCACTCA	AGGAACGAATCACACTTTTACCA	50.0	39.1	57.8	58.3	730-735 bp	1.0	59.3