Hotspot

rps16-trnQ-CAA

LSC · 6061-6420 · 360 bp

Back to Aphragmus

Pi 0.0615

MarkerSeek score 70.20

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 22
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	TCGAGCCAAGAGTGCCTATA	CTTACAGCTGCTTGCCAAAC	50.0	50.0	57.9	57.9	1090 bp	0.5	59.0
rps16-trnQ-CAA_p2	TCGAGCCAAGAGTGCCTATA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	527 bp	0.5	58.9
rps16-trnQ-CAA_p3	CACAGCAGGTAAGGTCCTTC	CTTACAGCTGCTTGCCAAAC	55.0	50.0	57.9	57.9	1021 bp	0.5	58.7
rps16-trnQ-CAA_p4	TCGAGCCAAGAGTGCCTATA	GATTCAAAAACGCGTAGGCC	50.0	50.0	57.9	58.1	1017 bp	0.5	58.6
rps16-trnQ-CAA_p5	TCGAGCCAAGAGTGCCTATA	GGATTCAAAAACGCGTAGGC	50.0	50.0	57.9	58.1	1018 bp	0.5	58.6