Hotspot

rpoA

Genome · 135435-137090 · 1656 bp

Back to Aphanochaete

Pi 0.268

MarkerSeek score 83.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1656
variable_sites: 417
pi: 0.268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TAGAAGGGTTAAGGCAGCCA	CACCCCCAACATTCCAAAGA	50.0	50.0	58.3	58.0	2883 bp	0.5	57.2
rpoA_p2	TAGAAGGGTTAAGGCAGCCA	ACAACGACTTTTTCCGGGAA	50.0	45.0	58.3	58.0	2957 bp	0.5	57.0
rpoA_p3	TAGAAGGGTTAAGGCAGCCA	AACAACAGAAGCCCACCAAT	50.0	45.0	58.3	57.9	2696 bp	0.5	56.6
rpoA_p4	AGCCAAGCTGCCTTAGTTTC	CACCCCCAACATTCCAAAGA	50.0	50.0	58.5	58.0	2868 bp	0.5	56.2
rpoA_p5	TAGAAGGGTTAAGGCAGCCA	AAACAACAGAAGCCCACCAA	50.0	45.0	58.3	58.1	2697 bp	0.5	56.2