Hotspot

petL-rpoC2

Genome · 122091-122746 · 656 bp

Back to Aphanochaete

Pi 0.338

MarkerSeek score 83.30

Primer available yes

Label petL-rpoC2

Feature Metadata

label_name: petL-rpoC2
feature_type: igs
region: Genome
length_bp: 656
variable_sites: 208
pi: 0.338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-rpoC2_p1	AACAAGCAATGTCTACGCCT	GCCATTAAACCACGCATAGC	45.0	50.0	57.8	57.9	2081 bp	0.5	57.6
petL-rpoC2_p2	AACAAGCAATGTCTACGCCT	ATCTGCCATTAAACCACGCA	45.0	45.0	57.8	58.2	2085 bp	0.5	57.2
petL-rpoC2_p3	AACAAGCAATGTCTACGCCT	TCTGCCATTAAACCACGCAT	45.0	45.0	57.8	58.2	2084 bp	0.5	57.2
petL-rpoC2_p4	AACAAGCAATGTCTACGCCT	ACCTGAGAAACATTCCCACG	45.0	50.0	57.8	57.8	2048 bp	0.5	57.0
petL-rpoC2_p5	AACAAGCAATGTCTACGCCT	CCTGAGAAACATTCCCACGT	45.0	50.0	57.8	57.8	2047 bp	0.5	57.0