Hotspot

psbB-psbT

Genome · 98643-99507 · 865 bp

Back to Aphanochaete

Pi 0.357

MarkerSeek score 82.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: Genome
length_bp: 865
variable_sites: 283
pi: 0.357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	CGTTATCAGTGGGACCAAGG	TCACAATACGAGGTGGTTCTC	55.0	47.6	58.0	57.4	1670 bp	0.5	47.1
psbB-psbT_p2	CGTTATCAGTGGGACCAAGG	TCTCACAATACGAGGTGGTTC	55.0	47.6	58.0	57.4	1672 bp	0.5	47.1
psbB-psbT_p3	CGTTATCAGTGGGACCAAGG	CACAATACGAGGTGGTTCTCT	55.0	47.6	58.0	57.4	1669 bp	0.5	47.0
psbB-psbT_p4	CGTTATCAGTGGGACCAAGG	CTCACAATACGAGGTGGTTCT	55.0	47.6	58.0	57.4	1671 bp	0.5	47.0
psbB-psbT_p5	TGGTGGCGAATTAGATGGTG	TCACAATACGAGGTGGTTCTC	50.0	47.6	58.0	57.4	1278 bp	0.5	47.0