Hotspot

rbcL-rps14

Genome · 74576-75147 · 572 bp

Back to Aphanochaete

Pi 0.379

MarkerSeek score 82.70

Primer available yes

Label rbcL-rps14

Feature Metadata

label_name: rbcL-rps14
feature_type: igs
region: Genome
length_bp: 572
variable_sites: 193
pi: 0.379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-rps14_p1	AGACTTAGCGCGTAAAGGTG	TTGGTGTGCCATTTCACGTA	50.0	45.0	58.0	58.0	963 bp	0.5	59.7
rbcL-rps14_p2	GCACATGCCAGCTTTAGTTG	TTGGTGTGCCATTTCACGTA	50.0	45.0	58.0	58.0	1113 bp	0.5	59.6
rbcL-rps14_p3	TGGTGATGTTATCCGTGCAG	TTGGTGTGCCATTTCACGTA	50.0	45.0	58.0	58.0	945 bp	0.5	59.6
rbcL-rps14_p4	GTACTCAAGCTCGTAACGCA	TTGGTGTGCCATTTCACGTA	50.0	45.0	58.0	58.0	988 bp	0.5	59.5
rbcL-rps14_p5	TGTACTCAAGCTCGTAACGC	TTGGTGTGCCATTTCACGTA	50.0	45.0	58.0	58.0	989 bp	0.5	59.5