Hotspot

psbK-psbL

Genome · 68692-69426 · 735 bp

Back to Aphanochaete

Pi 0.332

MarkerSeek score 83.10

Primer available yes

Label psbK-psbL

Feature Metadata

label_name: psbK-psbL
feature_type: igs
region: Genome
length_bp: 735
variable_sites: 218
pi: 0.332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbL_p1	CGGAGGCTTATGCTCCTTTT	TGGGCGTGAAACTACTTCTG	50.0	50.0	58.0	57.8	902 bp	0.5	58.3
psbK-psbL_p2	CGGAGGCTTATGCTCCTTTT	GGCGTGAAACTACTTCTGCT	50.0	50.0	58.0	58.2	900 bp	0.5	58.0
psbK-psbL_p3	CGGAGGCTTATGCTCCTTTT	TGTTTGTTTGGATTTGGGCG	50.0	45.0	58.0	57.8	916 bp	0.5	57.8
psbK-psbL_p4	CGGAGGCTTATGCTCCTTTT	TTTGTTTGGATTTGGGCGTG	50.0	45.0	58.0	57.8	914 bp	0.5	57.8
psbK-psbL_p5	CGGAGGCTTATGCTCCTTTT	GTTTGTTTGGATTTGGGCGT	50.0	45.0	58.0	57.8	915 bp	0.5	57.8