Hotspot

trnH(gug)-trnV(uac)

Genome · 47552-47894 · 343 bp

Back to Aphanochaete

Pi 0.484
MarkerSeek score 75.80
Primer available yes
Label trnH(gug)-trnV(uac)

Feature Metadata

label_name
trnH(gug)-trnV(uac)
feature_type
igs
region
Genome
length_bp
343
variable_sites
135
pi
0.484
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(gug)-trnV(uac)_p1 ACCCATTGCCTTACCACTTG CCAACTGAGTTATGGGCCT 50.0 52.6 58.1 56.7 393 bp 0.5 41.0
trnH(gug)-trnV(uac)_p2 CAACCCATTGCCTTACCACT CCAACTGAGTTATGGGCCT 50.0 52.6 58.1 56.7 395 bp 0.5 41.0
trnH(gug)-trnV(uac)_p3 CATGATGGAACCACAACCCA CCAACTGAGTTATGGGCCT 50.0 52.6 58.1 56.7 408 bp 0.5 41.0
trnH(gug)-trnV(uac)_p4 TTGCCTTACCACTTGGCTAC CCAACTGAGTTATGGGCCT 50.0 52.6 57.8 56.7 388 bp 0.5 40.1
trnH(gug)-trnV(uac)_p5 ACAACCCATTGCCTTACCAC CCAACTGAGTTATGGGCCT 50.0 52.6 58.4 56.7 396 bp 0.5 38.7