Hotspot

petD-rps4

Genome · 20863-22355 · 1493 bp

Back to Aphanochaete

Pi 0.336

MarkerSeek score 82.60

Primer available yes

Label petD-rps4

Feature Metadata

label_name: petD-rps4
feature_type: igs
region: Genome
length_bp: 1493
variable_sites: 452
pi: 0.336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps4_p1	TTCTCCAATTGCTGCTGGTT	ATTGTTCGTGATATGGCCCC	45.0	50.0	58.0	58.0	1763 bp	0.5	59.8
petD-rps4_p2	ACAGTTGTTGCGATTGGTCT	ATTGTTCGTGATATGGCCCC	45.0	50.0	58.0	58.0	1966 bp	0.5	59.6
petD-rps4_p3	TTCTCCAATTGCTGCTGGTT	ACGTCGTAGTGTAGGTTTGC	45.0	50.0	58.0	57.9	1743 bp	0.5	59.5
petD-rps4_p4	ACAGTTGTTGCGATTGGTCT	ACGTCGTAGTGTAGGTTTGC	45.0	50.0	58.0	57.9	1946 bp	0.5	59.3
petD-rps4_p5	CTGTTAAAAGCCCTGCTGGA	ATTGTTCGTGATATGGCCCC	50.0	50.0	58.1	58.0	1902 bp	0.5	59.1