primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
petD-rps4_p1	petD-rps4	1	TTCTCCAATTGCTGCTGGTT	ATTGTTCGTGATATGGCCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.019095	20863	22355	1763-1763	0.500000	59.8
petD-rps4_p2	petD-rps4	2	ACAGTTGTTGCGATTGGTCT	ATTGTTCGTGATATGGCCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.051425	20863	22355	1966-1966	0.500000	59.6
petD-rps4_p3	petD-rps4	3	TTCTCCAATTGCTGCTGGTT	ACGTCGTAGTGTAGGTTTGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.056446	20863	22355	1743-1743	0.500000	59.5
petD-rps4_p4	petD-rps4	4	ACAGTTGTTGCGATTGGTCT	ACGTCGTAGTGTAGGTTTGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.088776	20863	22355	1946-1946	0.500000	59.3
petD-rps4_p5	petD-rps4	5	CTGTTAAAAGCCCTGCTGGA	ATTGTTCGTGATATGGCCCC	20	20	50.0	50.0	58.1	58.0	11.9	0.0	0.109044	20863	22355	1902-1902	0.500000	59.1
rpoC1_p1	rpoC1	1	GCTATTTGCGTTTTATGCCGA	GAACGCATTTTTGGCCCTAC	21	20	42.9	50.0	57.9	58.0	0.0	0.0	1.068835	46101	46220	725-725	0.500000	51.4
rpoC1_p2	rpoC1	2	GCTATTTGCGTTTTATGCCGA	AATGCCAATACCTTGCACCA	21	20	42.9	45.0	57.9	58.1	0.0	0.0	1.123835	46101	46220	782-782	0.500000	51.0
rpoC1_p3	rpoC1	3	GCTATTTGCGTTTTATGCCGA	AACGCGTCCAGATTTGATGA	21	20	42.9	45.0	57.9	57.9	0.0	14.4	1.152941	46101	46220	899-899	0.500000	50.8
rpoC1_p4	rpoC1	4	GCTATTTGCGTTTTATGCCGA	ACGCGTCCAGATTTGATGAA	21	20	42.9	45.0	57.9	57.9	0.0	5.6	1.152941	46101	46220	898-898	0.500000	50.8
rpoC1_p5	rpoC1	5	GCTATTTGCGTTTTATGCCGA	TCTGTGAACGCATTTTTGGC	21	20	42.9	45.0	57.9	57.9	0.0	0.0	1.192374	46101	46220	730-730	0.500000	50.5
trnH(gug)-trnV(uac)_p1	trnH(gug)-trnV(uac)	1	ACCCATTGCCTTACCACTTG	CCAACTGAGTTATGGGCCT	20	19	50.0	52.6	58.1	56.7	0.0	4.5	2.372181	47552	47894	393-393	0.500000	41.0
trnH(gug)-trnV(uac)_p2	trnH(gug)-trnV(uac)	2	CAACCCATTGCCTTACCACT	CCAACTGAGTTATGGGCCT	20	19	50.0	52.6	58.1	56.7	0.0	4.5	2.372181	47552	47894	395-395	0.500000	41.0
trnH(gug)-trnV(uac)_p3	trnH(gug)-trnV(uac)	3	CATGATGGAACCACAACCCA	CCAACTGAGTTATGGGCCT	20	19	50.0	52.6	58.1	56.7	0.0	4.5	2.373555	47552	47894	408-408	0.500000	41.0
trnH(gug)-trnV(uac)_p4	trnH(gug)-trnV(uac)	4	TTGCCTTACCACTTGGCTAC	CCAACTGAGTTATGGGCCT	20	19	50.0	52.6	57.8	56.7	0.0	4.5	2.489739	47552	47894	388-388	0.500000	40.1
trnH(gug)-trnV(uac)_p5	trnH(gug)-trnV(uac)	5	ACAACCCATTGCCTTACCAC	CCAACTGAGTTATGGGCCT	20	19	50.0	52.6	58.4	56.7	0.0	4.5	2.667673	47552	47894	396-396	0.500000	38.7
trnM(cau)-trnfM(cau)_p1	trnM(cau)-trnfM(cau)	1	CGGTTTAGGCAATCGACTCA	ATATCGCTGGGTAGAGCAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.007088	48217	49561	1481-1481	0.500000	59.9
trnM(cau)-trnfM(cau)_p2	trnM(cau)-trnfM(cau)	2	CGGTTTAGGCAATCGACTCA	AGCCTGCTTAACTCAATCGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034421	48217	49561	1714-1714	0.500000	59.7
trnM(cau)-trnfM(cau)_p3	trnM(cau)-trnfM(cau)	3	CGGTTTAGGCAATCGACTCA	TTTCCAAAACCGGATGTCGT	20	20	50.0	45.0	58.0	58.0	0.0	9.5	0.034491	48217	49561	2026-2026	0.500000	59.7
trnM(cau)-trnfM(cau)_p4	trnM(cau)-trnfM(cau)	4	CGGTTTAGGCAATCGACTCA	TAGAACGCCGGTTTCCAAAA	20	20	50.0	45.0	58.0	58.0	0.0	1.9	0.040030	48217	49561	2037-2037	0.500000	59.7
trnM(cau)-trnfM(cau)_p5	trnM(cau)-trnfM(cau)	5	ACCCATTGCCTTACCACTTG	ATATCGCTGGGTAGAGCAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.079150	48217	49561	2119-2119	0.500000	59.4
trnS(gga)-rrs_p1	trnS(gga)-rrs	1	TCGAACCCTCGGTAGTTCTT	CTCTCAGTGTCAGTAACGGC	20	20	50.0	55.0	58.1	58.0	9.6	0.0	0.100887	61911	62518	1419-1419	0.500000	59.2
trnS(gga)-rrs_p2	trnS(gga)-rrs	2	TTCGAACCCTCGGTAGTTCT	CTCTCAGTGTCAGTAACGGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.100887	61911	62518	1420-1420	0.500000	59.2
trnS(gga)-rrs_p3	trnS(gga)-rrs	3	TTCAAAGCGAGCTTCCCATT	CTCTCAGTGTCAGTAACGGC	20	20	45.0	55.0	58.1	58.0	11.0	0.0	0.106328	61911	62518	2063-2063	0.500000	59.1
trnS(gga)-rrs_p4	trnS(gga)-rrs	4	TTTCAAAGCGAGCTTCCCAT	CTCTCAGTGTCAGTAACGGC	20	20	45.0	55.0	58.1	58.0	11.0	0.0	0.106328	61911	62518	2064-2064	0.500000	59.1
trnS(gga)-rrs_p5	trnS(gga)-rrs	5	TCGAACCCTCGGTAGTTCTT	TACGTCGTCTGACTTGCATG	20	20	50.0	50.0	58.1	58.0	9.6	0.0	0.107671	61911	62518	749-749	0.500000	59.1
psbK-psbL_p1	psbK-psbL	1	CGGAGGCTTATGCTCCTTTT	TGGGCGTGAAACTACTTCTG	20	20	50.0	50.0	58.0	57.8	13.4	0.0	0.207941	68692	69426	902-902	0.500000	58.3
psbK-psbL_p2	psbK-psbL	2	CGGAGGCTTATGCTCCTTTT	GGCGTGAAACTACTTCTGCT	20	20	50.0	50.0	58.0	58.2	13.4	0.0	0.247098	68692	69426	900-900	0.500000	58.0
psbK-psbL_p3	psbK-psbL	3	CGGAGGCTTATGCTCCTTTT	TGTTTGTTTGGATTTGGGCG	20	20	50.0	45.0	58.0	57.8	13.4	0.0	0.275337	68692	69426	916-916	0.500000	57.8
psbK-psbL_p4	psbK-psbL	4	CGGAGGCTTATGCTCCTTTT	TTTGTTTGGATTTGGGCGTG	20	20	50.0	45.0	58.0	57.8	13.4	0.0	0.275337	68692	69426	914-914	0.500000	57.8
psbK-psbL_p5	psbK-psbL	5	CGGAGGCTTATGCTCCTTTT	GTTTGTTTGGATTTGGGCGT	20	20	50.0	45.0	58.0	57.8	13.4	0.0	0.276879	68692	69426	915-915	0.500000	57.8
rbcL-rps14_p1	rbcL-rps14	1	AGACTTAGCGCGTAAAGGTG	TTGGTGTGCCATTTCACGTA	20	20	50.0	45.0	58.0	58.0	21.2	0.0	0.039126	74576	75147	963-963	0.500000	59.7
rbcL-rps14_p2	rbcL-rps14	2	GCACATGCCAGCTTTAGTTG	TTGGTGTGCCATTTCACGTA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044598	74576	75147	1113-1113	0.500000	59.6
rbcL-rps14_p3	rbcL-rps14	3	TGGTGATGTTATCCGTGCAG	TTGGTGTGCCATTTCACGTA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054060	74576	75147	945-945	0.500000	59.6
rbcL-rps14_p4	rbcL-rps14	4	GTACTCAAGCTCGTAACGCA	TTGGTGTGCCATTTCACGTA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058157	74576	75147	988-988	0.500000	59.5
rbcL-rps14_p5	rbcL-rps14	5	TGTACTCAAGCTCGTAACGC	TTGGTGTGCCATTTCACGTA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058157	74576	75147	989-989	0.500000	59.5
petB-psbI_p1	petB-psbI	1	TTTTACGAGGGGGTGTAGGT	AGCACCAATGTTCACATTCCT	20	21	50.0	42.9	58.0	58.1	0.0	2.0	1.148118	81906	82567	1314-1314	0.500000	50.8
petB-psbI_p2	petB-psbI	2	CCGTTGGTCTGCAAGTATGA	AGCACCAATGTTCACATTCCT	20	21	50.0	42.9	57.9	58.1	0.0	2.0	1.212863	81906	82567	1559-1559	0.500000	50.3
petB-psbI_p3	petB-psbI	3	CTATTCACCGTTGGTCTGCA	AGCACCAATGTTCACATTCCT	20	21	50.0	42.9	57.9	58.1	0.0	2.0	1.212863	81906	82567	1566-1566	0.500000	50.3
petB-psbI_p4	petB-psbI	4	GCAAGGTATTTCTGGTCCGT	AGCACCAATGTTCACATTCCT	20	21	50.0	42.9	57.9	58.1	0.0	2.0	1.228982	81906	82567	1193-1193	0.500000	50.2
petB-psbI_p5	petB-psbI	5	TCGTTACCATGGGATCAGGT	AGCACCAATGTTCACATTCCT	20	21	50.0	42.9	58.1	58.1	18.1	2.0	1.245427	81906	82567	1408-1408	0.500000	50.0
chlN_p1	chlN	1	AGGAATGTGAACATTGGTGCT	ATTCGTAAACTCGGCTCAGC	21	20	42.9	50.0	58.1	58.4	13.2	0.0	1.477980	83796	85313	2896-2896	0.500000	48.2
chlN_p2	chlN	2	AGGAATGTGAACATTGGTGCT	ACAAGACGCCACTAATTCGT	21	20	42.9	45.0	58.1	57.5	13.2	0.0	1.570166	83796	85313	2828-2828	0.500000	47.4
chlN_p3	chlN	3	GAGGAATGTGAACATTGGTGC	ATTCGTAAACTCGGCTCAGC	21	20	47.6	50.0	57.8	58.4	13.2	0.0	1.580387	83796	85313	2897-2897	0.500000	47.4
chlN_p4	chlN	4	AGGAATGTGAACATTGGTGCT	CGGAGCTGAGCCTATTTCAT	21	20	42.9	50.0	58.1	57.5	13.2	0.0	1.659821	83796	85313	2877-2877	0.500000	46.7
chlN_p5	chlN	5	GAGGAATGTGAACATTGGTGC	ACAAGACGCCACTAATTCGT	21	20	47.6	45.0	57.8	57.5	13.2	0.0	1.672574	83796	85313	2829-2829	0.500000	46.6
psbB-psbT_p1	psbB-psbT	1	CGTTATCAGTGGGACCAAGG	TCACAATACGAGGTGGTTCTC	20	21	55.0	47.6	58.0	57.4	0.0	0.0	1.611972	98643	99507	1670-1670	0.500000	47.1
psbB-psbT_p2	psbB-psbT	2	CGTTATCAGTGGGACCAAGG	TCTCACAATACGAGGTGGTTC	20	21	55.0	47.6	58.0	57.4	0.0	0.0	1.611972	98643	99507	1672-1672	0.500000	47.1
psbB-psbT_p3	psbB-psbT	3	CGTTATCAGTGGGACCAAGG	CACAATACGAGGTGGTTCTCT	20	21	55.0	47.6	58.0	57.4	0.0	0.0	1.618778	98643	99507	1669-1669	0.500000	47.0
psbB-psbT_p4	psbB-psbT	4	CGTTATCAGTGGGACCAAGG	CTCACAATACGAGGTGGTTCT	20	21	55.0	47.6	58.0	57.4	0.0	0.0	1.618778	98643	99507	1671-1671	0.500000	47.0
psbB-psbT_p5	psbB-psbT	5	TGGTGGCGAATTAGATGGTG	TCACAATACGAGGTGGTTCTC	20	21	50.0	47.6	58.0	57.4	0.0	0.0	1.624754	98643	99507	1278-1278	0.500000	47.0
ftsH_p1	ftsH	1	AGATTGGCATTCAAACGACT	AACGCGGTAACTTTGATGGA	20	20	40.0	45.0	55.6	57.8	11.6	12.5	2.527459	107330	107449	1043-1043	0.500000	39.8
ftsH_p2	ftsH	2	AGATTGGCATTCAAACGACTT	AACGCGGTAACTTTGATGGA	21	20	38.1	45.0	56.4	57.8	11.6	12.5	2.741820	107330	107449	1043-1043	0.500000	38.1
ftsH_p3	ftsH	3	AGATTGGCATTCAAACGACT	ACGCGGTAACTTTGATGGAT	20	20	40.0	45.0	55.6	57.6	11.6	12.5	2.751198	107330	107449	1042-1042	0.500000	38.0
ftsH_p4	ftsH	4	TGTAGATTGGCATTCAAACGAC	AACGCGGTAACTTTGATGGA	22	20	40.9	45.0	57.3	57.8	11.6	12.5	2.851360	107330	107449	1046-1046	0.500000	37.2
ftsH_p5	ftsH	5	ACTGTAGATTGGCATTCAAACG	AACGCGGTAACTTTGATGGA	22	20	40.9	45.0	57.3	57.8	11.6	12.5	2.858020	107330	107449	1048-1048	0.500000	37.1
petL-rpoC2_p1	petL-rpoC2	1	AACAAGCAATGTCTACGCCT	GCCATTAAACCACGCATAGC	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.304676	122091	122746	2081-2081	0.500000	57.6
petL-rpoC2_p2	petL-rpoC2	2	AACAAGCAATGTCTACGCCT	ATCTGCCATTAAACCACGCA	20	20	45.0	45.0	57.8	58.2	0.0	0.0	0.352121	122091	122746	2085-2085	0.500000	57.2
petL-rpoC2_p3	petL-rpoC2	3	AACAAGCAATGTCTACGCCT	TCTGCCATTAAACCACGCAT	20	20	45.0	45.0	57.8	58.2	0.0	0.0	0.352121	122091	122746	2084-2084	0.500000	57.2
petL-rpoC2_p4	petL-rpoC2	4	AACAAGCAATGTCTACGCCT	ACCTGAGAAACATTCCCACG	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.370008	122091	122746	2048-2048	0.500000	57.0
petL-rpoC2_p5	petL-rpoC2	5	AACAAGCAATGTCTACGCCT	CCTGAGAAACATTCCCACGT	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.370008	122091	122746	2047-2047	0.500000	57.0
rpoBb-rpl32_p1	rpoBb-rpl32	1	AAATCCGACGATGTGACAGG	ATAAAGAGTGGCTGGGAGAC	20	20	50.0	50.0	58.0	56.6	0.0	0.0	1.393472	133015	133617	1530-1530	0.500000	48.9
rpoBb-rpl32_p2	rpoBb-rpl32	2	ATAGGGACACCAGCCTCTTT	ATAAAGAGTGGCTGGGAGAC	20	20	50.0	50.0	58.0	56.6	0.0	0.0	1.410739	133015	133617	1464-1464	0.500000	48.7
rpoBb-rpl32_p3	rpoBb-rpl32	3	TGGGCAACGTTTAGGTGAAA	ATAAAGAGTGGCTGGGAGAC	20	20	45.0	50.0	57.9	56.6	0.0	0.0	1.437559	133015	133617	1612-1612	0.500000	48.5
rpoBb-rpl32_p4	rpoBb-rpl32	4	GTGAAATGGAAGTTTGGGCG	ATAAAGAGTGGCTGGGAGAC	20	20	50.0	50.0	57.9	56.6	0.0	0.0	1.447900	133015	133617	1598-1598	0.500000	48.4
rpoBb-rpl32_p5	rpoBb-rpl32	5	ACCTGCAGGATCTGTCACTA	ATAAAGAGTGGCTGGGAGAC	20	20	50.0	50.0	57.8	56.6	39.2	0.0	1.621411	133015	133617	1912-1912	0.500000	47.0
rpoA_p1	rpoA	1	TAGAAGGGTTAAGGCAGCCA	CACCCCCAACATTCCAAAGA	20	20	50.0	50.0	58.3	58.0	15.8	0.0	0.344332	135435	137090	2883-2883	0.500000	57.2
rpoA_p2	rpoA	2	TAGAAGGGTTAAGGCAGCCA	ACAACGACTTTTTCCGGGAA	20	20	50.0	45.0	58.3	58.0	15.8	5.1	0.377340	135435	137090	2957-2957	0.500000	57.0
rpoA_p3	rpoA	3	TAGAAGGGTTAAGGCAGCCA	AACAACAGAAGCCCACCAAT	20	20	50.0	45.0	58.3	57.9	15.8	0.0	0.422699	135435	137090	2696-2696	0.500000	56.6
rpoA_p4	rpoA	4	AGCCAAGCTGCCTTAGTTTC	CACCCCCAACATTCCAAAGA	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.469086	135435	137090	2868-2868	0.500000	56.2
rpoA_p5	rpoA	5	TAGAAGGGTTAAGGCAGCCA	AAACAACAGAAGCCCACCAA	20	20	50.0	45.0	58.3	58.1	15.8	0.0	0.476787	135435	137090	2697-2697	0.500000	56.2