Hotspot

psbN

LSC · 77513-77644 · 132 bp

Back to Aphanocalyx

Pi 0.0152

MarkerSeek score 61.20

Primer available yes

Label psbN

Feature Metadata

label_name: psbN
feature_type: gene
region: LSC
length_bp: 132
variable_sites: 2
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN_p1	TGGGAGGCTCATTACTTCAA	CCTAGATCCATTGTGGTCTTGA	45.0	45.5	56.1	57.2	186 bp	1.0	43.8
psbN_p2	TGGGAGGCTCATTACTTCAA	TCCTAGATCCATTGTGGTCTTG	45.0	45.5	56.1	57.2	187 bp	1.0	43.8
psbN_p3	TTGGGAGGCTCATTACTTCA	CCTAGATCCATTGTGGTCTTGA	45.0	45.5	56.1	57.2	187 bp	1.0	43.8
psbN_p4	TTGGGAGGCTCATTACTTCA	TCCTAGATCCATTGTGGTCTTG	45.0	45.5	56.1	57.2	188 bp	1.0	43.8
psbN_p5	TGAAGTAATGAGCCTCCCAA	CCTAGATCCATTGTGGTCTTGA	45.0	45.5	56.1	57.2	209 bp	1.0	43.7