Hotspot

rpl32-trnL-UAG

SSC · 117508-118878 · 1371 bp

Back to Aphanocalyx

Pi 0.0135

MarkerSeek score 56.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1371
variable_sites: 18
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGCAACAAATAAAAATATTGGAGT	AGCGTGTCTACCGATTTCAC	28.0	50.0	55.4	58.0	1427-1431 bp	1.0	43.0
rpl32-trnL-UAG_p2	GTGCAACAAATAAAAATATTGGAGT	CAGCGTGTCTACCGATTTCA	28.0	50.0	55.4	58.0	1428-1432 bp	1.0	43.0
rpl32-trnL-UAG_p3	GTGCAACAAATAAAAATATTGGAGT	TCCTAAGAGCAGCGTGTCTA	28.0	50.0	55.4	57.9	1437-1441 bp	1.0	43.0
rpl32-trnL-UAG_p4	GTGCAACAAATAAAAATATTGGAGT	CTAAGAGCAGCGTGTCTACC	28.0	55.0	55.4	57.8	1435-1439 bp	1.0	43.0
rpl32-trnL-UAG_p5	GTGCAACAAATAAAAATATTGGAGT	AAGATAAGATGCCCATGCCG	28.0	50.0	55.4	58.1	1493-1497 bp	1.0	42.9