Hotspot

clpP-psbB

LSC · 75098-75622 · 525 bp

Back to Aphanocalyx

Pi 0.00762

MarkerSeek score 61.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 525
variable_sites: 4
pi: 0.00762
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GGGGAAATACCACATGACCC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.9	58.2	657-673 bp	1.0	78.0
clpP-psbB_p2	GGGGAAATACCACATGACCC	AAAGCAAACGACCAGGATCA	55.0	45.0	57.9	57.7	698-714 bp	1.0	77.8
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	567-583 bp	1.0	77.3
clpP-psbB_p4	CTTTTGGAACACCAATGGGC	AAAGCAAACGACCAGGATCA	50.0	45.0	57.8	57.7	608-624 bp	1.0	77.0
clpP-psbB_p5	GGGGAAATACCACATGACCC	CAGAAAGCAAACGACCAGGA	55.0	50.0	57.9	58.4	701-717 bp	1.0	76.7