Hotspot

trnF-GAA-ndhJ

LSC · 51572-52304 · 733 bp

Back to Aphanocalyx

Pi 0.0109

MarkerSeek score 58.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 8
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	884-889 bp	1.0	77.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	848-853 bp	1.0	77.6
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	893-898 bp	1.0	77.2
trnF-GAA-ndhJ_p4	ATGTGTCAAGAATGGTCGGG	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	901-906 bp	1.0	77.0
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	872-877 bp	1.0	76.9