Hotspot

rps4-trnT-UGU

LSC · 49117-49562 · 446 bp

Back to Aphanocalyx

Pi 0.0115

MarkerSeek score 64.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 5
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	534-536 bp	1.0	79.3
rps4-trnT-UGU_p2	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	602-604 bp	1.0	78.8
rps4-trnT-UGU_p3	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	539-541 bp	1.0	78.6
rps4-trnT-UGU_p4	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	502-504 bp	1.0	78.6
rps4-trnT-UGU_p5	TTTTGAAACGAGGCCCTCTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	538-540 bp	1.0	78.3