Hotspot

atpH-atpI

LSC · 14381-15390 · 1010 bp

Back to Aphanocalyx

Pi 0.00992

MarkerSeek score 64.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 10
pi: 0.00992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1131-1291 bp	1.0	79.5
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1185-1345 bp	1.0	79.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1097-1257 bp	1.0	79.4
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1180-1340 bp	1.0	78.5
atpH-atpI_p5	CGCAATCCCTTCTACAGCTT	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1234-1394 bp	1.0	78.4