Hotspot

atpF-atpH

LSC · 13519-14134 · 616 bp

Back to Aphanocalyx

Pi 0.0052

MarkerSeek score 66.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 3
pi: 0.0052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	AGGCAGAGGGAAAAATACGG	50.0	50.0	58.1	57.6	766-800 bp	1.0	80.1
atpF-atpH_p2	CGTATGGCCAGTAACCCAAA	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	747-781 bp	1.0	80.0
atpF-atpH_p3	TTAAACCCGAAACTCCCAGC	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.1	57.5	770-804 bp	1.0	79.3
atpF-atpH_p4	CGTATGGCCAGTAACCCAAA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	751-785 bp	1.0	79.2
atpF-atpH_p5	TTAAACCCGAAACTCCCAGC	GCAGAGGGAAAAATACGGGG	50.0	55.0	58.1	58.6	764-798 bp	1.0	78.6