Hotspot

trnK-UUU-rps16

LSC · 4423-5352 · 930 bp

Back to Aphanocalyx

Pi 0.00878

MarkerSeek score 65.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 8
pi: 0.00878
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGCTGGTATGAACGAATCCA	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	58.1	58.0	1082-1102 bp	1.0	81.2
trnK-UUU-rps16_p2	TCGCTGGTATGAACGAATCC	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	57.8	58.0	1083-1103 bp	1.0	80.0
trnK-UUU-rps16_p3	CGCTGGTATGAACGAATCCA	GGCGGGTGTTTTTATCGAAC	50.0	50.0	58.1	57.7	1075-1095 bp	1.0	79.5
trnK-UUU-rps16_p4	TCGCTGGTATGAACGAATCC	GGCGGGTGTTTTTATCGAAC	50.0	50.0	57.8	57.7	1076-1096 bp	1.0	78.2
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	CAAAAAGGGCGGGTGTTTTT	50.0	45.0	58.6	58.0	1034-1054 bp	1.0	77.3