Hotspot

ycf1

IRb · 120493-121206 · 714 bp

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Pi 0.00373

MarkerSeek score 33.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 714
variable_sites: 4
pi: 0.00373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	TTTCTTTGCGGGTGAAGGAA	55.0	45.0	58.0	57.9	1574-1578 bp	1.0	82.1
ycf1_p2	CCGGAACTTCTTCGTAGTGG	TTTCTTTGCGGGTGAAGGAA	55.0	45.0	58.0	57.9	1573-1577 bp	1.0	82.1
ycf1_p3	GAGCTACTGAGGAACAACGG	TTTCTTTGCGGGTGAAGGAA	55.0	45.0	58.0	57.9	1669-1673 bp	1.0	82.0
ycf1_p4	CCGGAACTTCTTCGTAGTGG	TAGTAGGGGGTGGACGAATC	55.0	55.0	58.0	57.9	1544-1548 bp	1.0	82.0
ycf1_p5	GAGCTACTGAGGAACAACGG	TAGTAGGGGGTGGACGAATC	55.0	55.0	58.0	57.9	1640-1644 bp	1.0	81.9