Hotspot

ndhB-trnL(CAA)

IRa · 158638-159724 · 1087 bp

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Pi 0.0408

MarkerSeek score 82.10

Primer available yes

Label ndhB-trnL(CAA)

Feature Metadata

label_name: ndhB-trnL(CAA)
feature_type: igs
region: IRa
length_bp: 1087
variable_sites: 64
pi: 0.0408
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1147-1323 bp	1.0	86.1
ndhB-trnL(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1145-1321 bp	1.0	86.1
ndhB-trnL(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	1142-1318 bp	1.0	84.7
ndhB-trnL(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	1146-1322 bp	1.0	84.0
ndhB-trnL(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	1144-1320 bp	1.0	83.9