Hotspot

rps15-ycf1

SSC · 137139-138218 · 1080 bp

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Pi 0.0161
MarkerSeek score 70.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
1080
variable_sites
24
pi
0.0161
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCGACAGACCCCTTGTTTTC AGCTTGTATGAATCGGTATTGGT 50.0 39.1 57.8 58.0 1269-1347 bp 1.0 59.2
rps15-ycf1_p2 CGACAGACCCCTTGTTTTCT AGCTTGTATGAATCGGTATTGGT 50.0 39.1 57.7 58.0 1268-1346 bp 1.0 59.1
rps15-ycf1_p3 TCGACAGACCCCTTGTTTTC TGGTAGTCGTTTCAGTATGTCA 50.0 40.9 57.8 56.9 1233-1311 bp 1.0 58.5
rps15-ycf1_p4 CGACAGACCCCTTGTTTTCT TGGTAGTCGTTTCAGTATGTCA 50.0 40.9 57.7 56.9 1232-1310 bp 1.0 58.5
rps15-ycf1_p5 TCGACAGACCCCTTGTTTTC GCTTGTATGAATCGGTATTGGT 50.0 40.9 57.8 56.6 1268-1346 bp 1.0 56.1