Hotspot

ndhD-psaC

SSC · 128755-128867 · 113 bp

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Pi 0.0177

MarkerSeek score 63.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 3
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAAGAGCCCGCGAATATAGG	GCATGAGACAACTCGCAGTA	55.0	50.0	58.0	58.0	236 bp	1.0	81.0
ndhD-psaC_p2	GGAAGAGCCCGCGAATATAG	GCATGAGACAACTCGCAGTA	55.0	50.0	58.0	58.0	237 bp	1.0	81.0
ndhD-psaC_p3	GAAGAGCCCGCGAATATAGG	AGATGTGAATCCGCTTGTCC	55.0	50.0	58.0	58.0	289 bp	1.0	80.6
ndhD-psaC_p4	GGAAGAGCCCGCGAATATAG	AGATGTGAATCCGCTTGTCC	55.0	50.0	58.0	58.0	290 bp	1.0	80.6
ndhD-psaC_p5	GAAGAGCCCGCGAATATAGG	TATGGCATGAGACAACTCGC	55.0	50.0	58.0	58.1	240 bp	1.0	80.5