Hotspot

rrn5-trnR(ACG)

IRb · 119080-119329 · 250 bp

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Pi 0.00933
MarkerSeek score 58.70
Primer available yes
Label rrn5-trnR(ACG)

Feature Metadata

label_name
rrn5-trnR(ACG)
feature_type
igs
region
IRb
length_bp
250
variable_sites
3
pi
0.00933
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR(ACG)_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 333-334 bp 1.0 78.7
rrn5-trnR(ACG)_p2 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 368-369 bp 1.0 78.1
rrn5-trnR(ACG)_p3 ACCAATCCATCCCGAACTTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 371-372 bp 1.0 78.1
rrn5-trnR(ACG)_p4 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 369-370 bp 1.0 78.1
rrn5-trnR(ACG)_p5 CACCAATCCATCCCGAACTT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 372-373 bp 1.0 78.1