Hotspot

ycf4-cemA

LSC · 69636-70861 · 1226 bp

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Pi 0.00778

MarkerSeek score 62.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1226
variable_sites: 14
pi: 0.00778
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AAAAGCTGCGGAATTAGCCT	TGTGTCTCAGGGTACTCCTT	45.0	50.0	58.2	57.3	1276-1304 bp	1.0	75.9
ycf4-cemA_p2	TATTTCTCGCGCGTACCAAT	TGTGTCTCAGGGTACTCCTT	45.0	50.0	57.8	57.3	1257-1285 bp	1.0	75.5
ycf4-cemA_p3	CTATTTCTCGCGCGTACCAA	TGTGTCTCAGGGTACTCCTT	50.0	50.0	58.4	57.3	1258-1286 bp	1.0	73.6
ycf4-cemA_p4	AAAAGCTGCGGAATTAGCCT	TGTGTCTCAGGGTACTCCTTT	45.0	47.6	58.2	58.0	1276-1304 bp	1.0	73.2
ycf4-cemA_p5	TATTTCTCGCGCGTACCAAT	TGTGTCTCAGGGTACTCCTTT	45.0	47.6	57.8	58.0	1257-1285 bp	1.0	72.9