Hotspot

trnF(GAA)-ndhJ

LSC · 54936-56331 · 1396 bp

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Pi 0.0114

MarkerSeek score 63.70

Primer available yes

Label trnF(GAA)-ndhJ

Feature Metadata

label_name: trnF(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 1396
variable_sites: 21
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1506-1538 bp	1.0	83.7
trnF(GAA)-ndhJ_p2	CGATGCATGGAAAATGGTCG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	1561-1593 bp	1.0	83.2
trnF(GAA)-ndhJ_p3	GCGATGCATGGAAAATGGTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1562-1594 bp	1.0	82.8
trnF(GAA)-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1549-1581 bp	1.0	82.4
trnF(GAA)-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	1548-1580 bp	1.0	82.4