Hotspot

trnK(UUU)-rps16

LSC · 4673-5382 · 710 bp

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Pi 0.00195
MarkerSeek score 63.60
Primer available yes
Label trnK(UUU)-rps16

Feature Metadata

label_name
trnK(UUU)-rps16
feature_type
igs
region
LSC
length_bp
710
variable_sites
2
pi
0.00195
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(UUU)-rps16_p1 CCGCACTTAAAAGCCGAGTA AAAGGAGCTCAACCCACAAG 50.0 50.0 58.3 58.0 794-817 bp 1.0 80.6
trnK(UUU)-rps16_p2 CCGCACTTAAAAGCCGAGTA AGCTCAACCCACAAGAACTG 50.0 50.0 58.3 58.0 789-812 bp 1.0 80.5
trnK(UUU)-rps16_p3 CATAGCCGCACTTAAAAGCC AAAGGAGCTCAACCCACAAG 50.0 50.0 57.5 58.0 799-822 bp 1.0 79.1
trnK(UUU)-rps16_p4 CATAGCCGCACTTAAAAGCC AGCTCAACCCACAAGAACTG 50.0 50.0 57.5 58.0 794-817 bp 1.0 79.0
trnK(UUU)-rps16_p5 CCGCACTTAAAAGCCGAGTA GAGCTCAACCCACAAGAACT 50.0 50.0 58.3 57.7 790-813 bp 1.0 78.6