Hotspot

psaB

LSC · 36061-38265 · 2205 bp

Back to Antirrhinum

Pi 0.00068

MarkerSeek score 57.80

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 3
pi: 0.00068
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GGCCTTCTCTCGATCGAAAG	ACAACATGGGCGTTCTTCTT	55.0	45.0	58.2	58.0	2330 bp	1.0	78.7
psaB_p2	AAAAAGACGGGCCTTCTCTC	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.8	58.0	2339 bp	1.0	78.5
psaB_p3	GGCCTTCTCTCGATCGAAAG	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.2	58.1	2380 bp	1.0	78.2
psaB_p4	GGCCTTCTCTCGATCGAAAG	GGGCGTTCTTCTTAGCAAGA	55.0	50.0	58.2	57.9	2323 bp	1.0	78.1
psaB_p5	AAAAAGACGGGCCTTCTCTC	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.8	58.1	2389 bp	1.0	77.9