Hotspot

matK

LSC · 1900-3576 · 1677 bp

Back to Antirrhinum

Pi 0.00318

MarkerSeek score 60.60

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1677
variable_sites: 10
pi: 0.00318
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTTCCAAGGTCTCAGAATTG	TCAAAAGAGCGATTGGGTTT	40.0	40.0	53.1	55.9	1775 bp	1.0	40.5
matK_p2	TCAGAATAGTGGAAATCCCA	TCAAAAGAGCGATTGGGTTT	40.0	40.0	53.3	55.9	1832 bp	1.0	40.4
matK_p3	TTCAGAATAGTGGAAATCCCA	TCAAAAGAGCGATTGGGTTT	38.1	40.0	54.2	55.9	1833 bp	1.0	40.4
matK_p4	TCAGAATAGTGGAAATCCCA	TTCCGAAATCAAAAGAGCGA	40.0	40.0	53.3	55.7	1840 bp	1.0	40.4
matK_p5	TCAGAATAGTGGAAATCCCA	TCCGAAATCAAAAGAGCGATTG	40.0	40.9	53.3	57.7	1839 bp	1.0	40.4