Hotspot

rps15-ycf1

SSC · 121875-122320 · 446 bp

Back to Antirrhinum

Pi 0.00644

MarkerSeek score 37.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 446
variable_sites: 5
pi: 0.00644
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGAGTCATTCTTCCATGCA	ATGCGAATAATGGCAGTCGT	50.0	45.0	58.0	58.0	873-879 bp	1.0	80.6
rps15-ycf1_p2	ATCAGTCGAAGAACACCGTG	ATGCGAATAATGGCAGTCGT	50.0	45.0	57.9	58.0	969-975 bp	1.0	80.2
rps15-ycf1_p3	CACCGTCGAGAGTAACGATC	ATGCGAATAATGGCAGTCGT	55.0	45.0	57.9	58.0	986-992 bp	1.0	80.0
rps15-ycf1_p4	CCGAGTCATTCTTCCATGCA	CAGATGGAACGAGGCTTTGA	50.0	50.0	58.0	57.9	1520-1526 bp	1.0	79.9
rps15-ycf1_p5	CCGAGTCATTCTTCCATGCA	TCAGATGGAACGAGGCTTTG	50.0	50.0	58.0	57.9	1521-1527 bp	1.0	79.9